Effect of Uighur medicine abnormal savda munzip on hypertrophic scar and transforming growth factor beta/Smad signal transduction pathway

doi:10.3969/j.issn.2095-4344.2014.46.003

Abstract

Abstract:

BACKGROUND: Previous observations have suggested that abnormal savda munziq has a possible anti-scaring effect. However, the precise pathological mechanism still remains unknown. Here, it is considered whether abnormal savda munziq can alleviate the formation of hypertrophic scar by affecting the expression of transform growth factor β (TGF-β)/Smad signal transduction pathway.

OBJECTIVE: To evaluate the in vitro effect of abnormal savda munziq on the proliferation and TGF-β/Smad signal transduction pathway in hypertrophic scar fibroblasts.

METHODS: Hypertrophic scar fibroblasts were divided into six groups to receive different treatments as group A (blank control group), groups B-E (0.2, 0.4, 0.6, 0.8, 1.0 g/L abnormal savda munziq). Each group contained six specimens. After intervention, the MTT test, flow cytometry and immunocytochemistry methods were used to detect the alteration of cell proliferation and TGF-β/Smad signal transduction pathway.

RESULTS AND CONCLUSION: The inhibition effect of abnormal savda munziq (0.2-0.8 g/L) on the fibroblasts enhanced in a concentration-dependent manner than the blank control group (P < 0.05). The immunocytochemistry results showed that the expression of Smad7 increased while expression of TGF-β1 decreased in hypertrophic scar fibroblasts with abnormal savda munziq. These findings suggest that the reduction on the proliferation caused by abnormal savda munziq could be attributed to its effects on TGF-β/Smad signal transduction pathway. Abnormal savda munziq could be used as an effective drug for treatment of hypertrophic scar.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: cicatrix, fibroblasts, transforming growth factor beta1

CLC Number:

R318

Li Nan, Kong Meng-long, Ma Tao, Li Jia-fu, Ma Shao-lin. Effect of Uighur medicine abnormal savda munzip on hypertrophic scar and transforming growth factor beta/Smad signal transduction pathway [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(46): 7391-7395.

Figures/Tables 2

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